HPLC Column, RP PFP, 3um, 2.1mm ID x 150mm Length, 100A. Cogent Brand. 1 EA.
Additional Info:
68322-15P-2 Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for complex separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.
The column chemistry provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it particularly effective for halogenated, aromatic, and polar analytes. It also serves as a suitable alternate column for USP methods where PFP selectivity is required.
150 mm Length – Maximum Resolution for Complex Separations
The 150 mm length is ideal when maximum resolving power is required, especially for complex mixtures or closely related compounds.
Highest resolution compared to shorter column lengths
Improved separation of structurally similar analytes
Increased peak capacity for demanding methods
✅ Recommended for complex samples and methods requiring maximum separation performance
Flow Rate & System Requirements (2.1 mm ID, 3 µm)
⚠️ Designed for LC‑MS and low‑flow applications; requires precise low‑flow system capability
Typical flow rate range: ~0.2 – 0.3 mL/min
Higher efficiency compared to 5 µm columns
Increased backpressure due to smaller particle size
Ideal for LC‑MS sensitivity and reduced solvent consumption
✅ Important: Standard HPLC systems must be capable of stable, accurate low flow rates—this is not universal across all systems
✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection
Why Choose PFP (F5) for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with fluorinated and halogenated analytes
Improved selectivity compared to C18 phases
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility in method development
Versatile 100 Å Pore Structure
Suitable for small to moderately sized molecules
Balanced retention and efficiency
Cogent RP PFP™ Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry
High Efficiency (3 µm Particles)
More efficient than 5 µm columns
Sharper peaks and improved resolution
High Purity Silica
Consistent, reproducible chromatographic performance
Long column lifetime
Typical Applications
Halogenated compound analysis
Aromatic compound separations
Polar compound retention
Pharmaceutical and environmental testing
USP alternate method development
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Designed for low‑flow and LC‑MS HPLC systems (2.1 mm ID)
Higher backpressure than larger particle sizes
Requires accurate low‑flow pump performance
Always use 0.2 µm filtration before injection